Use of monoclonal antibodies to analyse the expression of a multi-tubulin family.

@article{Birkett1985UseOM,
  title={Use of monoclonal antibodies to analyse the expression of a multi-tubulin family.},
  author={Christopher R. Birkett and Katie Eloise Foster and Linda A. Johnson and Keith Gull},
  journal={FEBS letters},
  year={1985},
  volume={187 2},
  pages={211-8}
}
We have used a panel of monoclonal antibodies in a study of the expression of multiple tubulins in Physarum polycephalum. Three anti-beta-tubulin monoclonal antibodies, DM1B, DM3B3 and KMX-1 all reacted with the beta 1-tubulin isotypes expressed in both myxamoebae and plasmodia. However, these antibodies showed a spectrum of reduced reactivity with the plasmodial beta 2-tubulin isotype - the competence of recognition of this isotype was graded DM1B greater than KMX-1 greater than DM3B3. The… CONTINUE READING

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