Use of molecular probes to assess geographic distribution of Pfiesteria species.

@article{Rublee2001UseOM,
  title={Use of molecular probes to assess geographic distribution of Pfiesteria species.},
  author={Parke A. Rublee and Jason W. Kempton and Eric Schaefer and Carol Allen and Joan S Harris and David W. Oldach and Holly A. Bowers and Torstein Tengs and Joann M. Burkholder and Howard B. Glasgow},
  journal={Environmental Health Perspectives},
  year={2001},
  volume={109},
  pages={765 - 767}
}
We have developed multiple polymerase chain reaction (PCR)-based methods for the detection of Pfiesteria sp. in cultures and environmental samples. More than 2,100 water and sediment samples from estuarine sites of the U.S. Atlantic and gulf coasts were assayed for the presence of Pfiesteria piscicida Steidinger & Burkholder and Pfiesteria shumwayae Glasgow & Burkholder by PCR probing of extracted DNA. Positive results were found in about 3% of samples derived from routine monitoring of coastal… CONTINUE READING