Use of molecular beacons for rapid, real-time, quantitative monitoring of cytotoxic T-lymphocyte epitope mutations in simian immunodeficiency virus.

@article{Peyerl2005UseOM,
  title={Use of molecular beacons for rapid, real-time, quantitative monitoring of cytotoxic T-lymphocyte epitope mutations in simian immunodeficiency virus.},
  author={Fred W. Peyerl and Dan H Barouch and Heidi S. Bazick and Edwin R Manuel and Norman L. Letvin},
  journal={Journal of clinical microbiology},
  year={2005},
  volume={43 9},
  pages={4773-9}
}
Immune pressure on lentiviruses exerted by cytotoxic T lymphocytes (CTL) selects for virus CTL epitope mutations. Currently employed methods for monitoring emerging CTL epitope mutations rely on the labor-intensive and time-consuming techniques of virus population or clonal sequencing. Here we describe the development of a high-throughput quantitative reverse transcription-PCR assay that facilitates large-scale CTL epitope monitoring. This approach utilizes both sequence-specific molecular… CONTINUE READING