Use of mRNA expression to detect the induction of drug metabolising enzymes in rat and human hepatocytes.

@article{Richert2009UseOM,
  title={Use of mRNA expression to detect the induction of drug metabolising enzymes in rat and human hepatocytes.},
  author={Lysiane Richert and Gregor Tuschl and Catherine Abadie and Nadine Blanchard and Dumrongsak Pekthong and Georges Mantion and J Weber and Stefan M{\"u}ller},
  journal={Toxicology and applied pharmacology},
  year={2009},
  volume={235 1},
  pages={86-96}
}
It is important to investigate the induction of cytochrome P450 (CYP) enzymes by drugs. The most relevant end point is enzyme activity; however, this requires many cells and is low throughput. We have compared the CYP1A, CYP2B and CYP3A induction response to eight inducers in rat and human hepatocytes using enzyme activities (CYP1A2 (ethoxyresorufin), 2B (benzoxyresorufin for rat and bupropion for human) and CYP3A (testosterone)) and Taqman Low Density Array (TLDA) analysis. There was a good… CONTINUE READING
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