Use of kinetic plots for the optimization of the separation time in ultra-high-pressure LC.

@article{Eeltink2010UseOK,
  title={Use of kinetic plots for the optimization of the separation time in ultra-high-pressure LC.},
  author={Sebastiaan Eeltink and Wim M C Decrop and Frank Steiner and Mario Ursem and Deirdre Cabooter and Gert Desmet and Wim T h Kok},
  journal={Journal of separation science},
  year={2010},
  volume={33 17-18},
  pages={2629-35}
}
The kinetic-plot approach, in which experimental t(0) and N-values are extrapolated to the performance at maximum system pressure by increasing the column length, was validated by coupling 250×3 mm columns packed with 3 μm particles. The extra-column volume introduced by coupling columns could be neglected with respect to the peak volumes. Plate numbers of up to 132,000 were experimentally achieved by coupling four columns. The maximum deviation between the experimental and predicted plate… CONTINUE READING

From This Paper

Topics from this paper.

Similar Papers

Loading similar papers…