Use of intracellular pH and annexin-V flow cytometric assays to monitor apoptosis and its suppression by bcl-2 over-expression in hybridoma cell culture.

@article{Ishaque1998UseOI,
  title={Use of intracellular pH and annexin-V flow cytometric assays to monitor apoptosis and its suppression by bcl-2 over-expression in hybridoma cell culture.},
  author={Armana Ishaque and Mohamed Al-Rubeai},
  journal={Journal of immunological methods},
  year={1998},
  volume={221 1-2},
  pages={43-57}
}
Accurate identification and quantitation of apoptosis is essential for developing efficient strategies for optimisation of culture viability and productivity in cell lines of industrial significance. We have examined the possibility of using carboxy-seminaphthorhodafluor-1-acetoxymethylester (carboxy SNARF-1-AM), a pH sensitive fluoroprobe and FITC-labelled annexin V (AV), a probe specific to phosphatidylserine exposed on the surface of apoptotic cells, to monitor apoptosis and to determine the… CONTINUE READING

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