Use of internally controlled real-time genome amplification for detection of variola virus and other orthopoxviruses infecting humans.

@article{Fedele2006UseOI,
  title={Use of internally controlled real-time genome amplification for detection of variola virus and other orthopoxviruses infecting humans.},
  author={Cesare Giovanni Fedele and Anabel Negredo and Francisca Molero and M{\'a}ria Paz S{\'a}nchez-Seco and Antonio Tenorio},
  journal={Journal of clinical microbiology},
  year={2006},
  volume={44 12},
  pages={4464-70}
}
Smallpox, once a devastating disease caused by Variola virus, a member of the Orthopoxvirus genus, was eradicated in 1980. However, the importance of variola virus infections has been stressed widely in the last few years, particularly following recent social events in the world. Today, variola virus is considered to be one of the most significant agents with potential use as a biological weapon. In this study we developed an internally controlled real-time PCR assay for rapid detection and… CONTINUE READING

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