Use of intermediate partitioning to calculate intrinsic isotope effects for the reaction catalyzed by malic enzyme.

@article{Grissom1985UseOI,
  title={Use of intermediate partitioning to calculate intrinsic isotope effects for the reaction catalyzed by malic enzyme.},
  author={Charles B Grissom and W. Wallace Cleland},
  journal={Biochemistry},
  year={1985},
  volume={24 4},
  pages={944-8}
}
For those enzymes that proceed via a stepwise reaction mechanism with a discrete chemical intermediate and where deuterium and 13C isotope effects are on separate steps, a new method has been developed to solve for the intrinsic deuterium and 13C kinetic isotope effects that relies on directly observing the partitioning of the intermediate between the forward and reverse directions. This observed partitioning ratio, along with the values of the primary deuterium, tritium, and 13C kinetic… CONTINUE READING