Use of inter-simple sequence repeat (ISSR) markers for discrimination between and within species of blackflies (Diptera: Simuliidae)

@article{Duinsk2006UseOI,
  title={Use of inter-simple sequence repeat (ISSR) markers for discrimination between and within species of blackflies (Diptera: Simuliidae)},
  author={Roman Du{\vs}insk{\'y} and Mat{\'u}{\vs} K{\'u}dela and Viera Stloukalov{\'a} and Ladislav Jedli{\vc}ka},
  journal={Biologia},
  year={2006},
  volume={61},
  pages={299-304}
}
The present study is the first report of fingerprinting in blackflies (Diptera: Simuliidae), using inter-simple sequence repeat (ISSR) markers. Among five primers tested, three tetranucleotide repeat primers ((GACA)4, (ACTG)4, (ACAG)4) generated a high proportion of polymorphic bands. Seven species representing various genera, subgenera or species groups were compared. No similar profiles were found. Intraspecific and interspecific banding patterns were analysed for two species in the… CONTINUE READING

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