Use of immobilized enzymes in drug metabolism studies

  title={Use of immobilized enzymes in drug metabolism studies},
  author={Deanne M. Dulik and Catherine Fenselau},
  journal={The FASEB Journal},
  pages={2235 - 2240}
The immobilization of drug‐metabolizing enzymes onto polymeric supports offers several advantages over use of conventional microsomal or soluble enzyme preparations. These include increased storage stability, facilitated separation of products from the incubation mixture, the ability to recover and reuse the enzyme catalyst, and in many cases, stabilization of the tertiary structure of membrane‐bound enzymes. Attachment of the protein to the solid support may be accomplished by adsorption… 

Immobilization of Glutathione-s-transferase Within Cross-Linked Gelatin Cylindrical Molds

  • F. Zihnioglu
  • Biology, Chemistry
    Artificial cells, blood substitutes, and immobilization biotechnology
  • 2003
Rabbit liver cytosolic glutathione‐s‐transferase was immobilized in cross‐linked gelatin cylindrical molds and found suitable with a high yield of 2,4‐dinitrophenyl‐GSH conjugate formation for studying the detoxification by conjugation in vitro.

Reactions on Immobilized Biocatalysts

This article characterizes the characterization of Immobilized Biocatalysts as well as investigating the applications of immobilization and biotransformation in various industrial processes.

In vitro metabolism of the antianxiety drug buspirone as a predictor of its metabolism in vivo.

Hepatocytes and phenobarbital- induced rat liver microsomes were better predictors of in vivo metabolism of buspirone than non-induced rat livermicrosomes and these in vitro systems should provide excellent models for studying the metabolism of other azaspirodecanedione-containing drugs.

Immobilized enzymes as potent antibiofilm agent

Immobilization of amylase, cellobiohydrolase, pectinase, subtilisin A and β‐N‐acetyl‐glucosaminidase (DspB) are proved to be most effective in inhibition of biofilm formation and removal of matured biofilm than their free forms.

Substrate channeling and enzyme complexes for biotechnological applications.

Conjugation of polychlorinated agrochemical sulphoxides and sulphones by glutathione.

Qualitative and quantitative differences were observed in product distributions between species and between microsomal and cytosolic protein.



The application of immobilized enzymes in an artificial liver support system.

A novel method of extracorporeal support for fulminant liver failure is reported whereby the most important detoxification processes of the liver are reproduced in an enzyme reactor. Most of the

Immobilization of Ligandin on Hollow Fiber Reactors

The aim of this work was to immobilize ligandin to the inner surface of a hollow fiber hemodialyzer in order to verify the possibility of designing an enzymatically active reactor with unchanged dialytic performance; toxic hydrophobic agents may be dialyzed through this bioreactor immediately after the enzymatic attack as hydrophilic non-noxious compounds.

Species-dependent glucuronidation of drugs by immobilized rabbit, rhesus monkey, and human UDP-glucuronyltransferases.

  • D. DulikC. Fenselau
  • Biology, Chemistry
    Drug metabolism and disposition: the biological fate of chemicals
  • 1987
Differences in the in vitro product formation paralleled in vivo species differences for the three drugs studied, and the same glucuronides were produced by immobilized human liver microsomes as have been found to be formed in vivo from theThree drugs studied.

Use of coimmobilized multienzyme systems as models for in vivo processes.

  • M. Deluca
  • Biology
    Current topics in cellular regulation
  • 1984

Characterization of rat-liver microsomal glutathione S-transferase activity.

Considerations indicate that the microsomal glutathione S-transferase(s) is distinct from the cytoplasmic enzymes catalyzing similar reactions, and the possibility of activating it through attack on a sulfhydryl group may represent an important physiological response to certain xenobiotics.

[Interaction of the components of the cytochrome P-450 monooxygenase system from liver microsomes. I. Immobilization of the solubilized and partially purified protein components].

It was concluded that the activity of the cytochrome P-450 monooxygenases is influenced by some kind of self-assembly.

Immobilized glucuronosyltransferase for the synthesis of conjugates.

The agarose-bound UDPglucuronosyltransferase is successfully used in the synthesis of p-nitrophenyl glucuronide in an overall yield of 50-70% and offers an easy and convenient synthetic tool for various drug glucuronides.

Simultaneous immobilization of cytochrome P-450 and glucuronyltransferase for synthesis of drug metabolites.

Cytochrome P-450, NADPH-cytochrome P-450 reductase, and glucuronyltransferase were immobilized simultaneously on cyanogen bromide-activated Sepharose from phenobarbital-induced rabbit liver

Large Agarose Beads for Extracorporeal Detoxification Systems 1. Preparation and some properties and applications of the large agarose beads in haemoperfusion

A method is reported by which agarose beads of diameter 1000 to 10000 microns can be prepared from Sepharose(R) 4B (native bead diameter 40 to 190 microns). Haemoperfusion experiments indicate that