Use of immobilized enzymes in drug metabolism studies

@article{Dulik1988UseOI,
  title={Use of immobilized enzymes in drug metabolism studies},
  author={Deanne M. Dulik and Catherine Fenselau},
  journal={The FASEB Journal},
  year={1988},
  volume={2},
  pages={2235 - 2240}
}
The immobilization of drug‐metabolizing enzymes onto polymeric supports offers several advantages over use of conventional microsomal or soluble enzyme preparations. These include increased storage stability, facilitated separation of products from the incubation mixture, the ability to recover and reuse the enzyme catalyst, and in many cases, stabilization of the tertiary structure of membrane‐bound enzymes. Attachment of the protein to the solid support may be accomplished by adsorption… 

Immobilization of Glutathione-s-transferase Within Cross-Linked Gelatin Cylindrical Molds

  • F. Zihnioglu
  • Biology, Chemistry
    Artificial cells, blood substitutes, and immobilization biotechnology
  • 2003
Rabbit liver cytosolic glutathione‐s‐transferase was immobilized in cross‐linked gelatin cylindrical molds and found suitable with a high yield of 2,4‐dinitrophenyl‐GSH conjugate formation for studying the detoxification by conjugation in vitro.

Reactions on Immobilized Biocatalysts

This article characterizes the characterization of Immobilized Biocatalysts as well as investigating the applications of immobilization and biotransformation in various industrial processes.

In vitro metabolism of the antianxiety drug buspirone as a predictor of its metabolism in vivo.

Hepatocytes and phenobarbital- induced rat liver microsomes were better predictors of in vivo metabolism of buspirone than non-induced rat livermicrosomes and these in vitro systems should provide excellent models for studying the metabolism of other azaspirodecanedione-containing drugs.

Immobilized enzymes as potent antibiofilm agent

Immobilization of amylase, cellobiohydrolase, pectinase, subtilisin A and β‐N‐acetyl‐glucosaminidase (DspB) are proved to be most effective in inhibition of biofilm formation and removal of matured biofilm than their free forms.

Substrate channeling and enzyme complexes for biotechnological applications.

Conjugation of polychlorinated agrochemical sulphoxides and sulphones by glutathione.

Qualitative and quantitative differences were observed in product distributions between species and between microsomal and cytosolic protein.

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  • Biology, Chemistry
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Differences in the in vitro product formation paralleled in vivo species differences for the three drugs studied, and the same glucuronides were produced by immobilized human liver microsomes as have been found to be formed in vivo from theThree drugs studied.

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