Use of hollow fiber systems for rapid and direct scale up of antibody production from hybridoma cell lines cultured in CL-1000 flasks using BD Cell MAb medium

Abstract

The combination of BD Cell MAb medium with the CL-1000 flask is increasingly being used to generate a few hundred milligram of antibody for early stage research projects. Cells are inoculated at 2 million per ml, and the antibody is harvested after 15 days or when the antibody concentration reaches above 10 mg ml−1, whichever comes first. Currently, there… (More)
DOI: 10.1023/B:CYTO.0000015842.54956.24

6 Figures and Tables

Topics

  • Presentations referencing similar topics