Use of group-specific primers and the polymerase chain reaction for the detection and identification of luteoviruses.

@article{Robertson1991UseOG,
  title={Use of group-specific primers and the polymerase chain reaction for the detection and identification of luteoviruses.},
  author={Nancy L. Robertson and R. French and Stewart Gray},
  journal={The Journal of general virology},
  year={1991},
  volume={72 ( Pt 6)},
  pages={1473-7}
}
A general diagnostic assay for a number of distinct luteoviruses was developed using the polymerase chain reaction (PCR) and restriction enzyme analysis. Two minimally degenerate, group-specific primers were derived from previously published RNA sequences of three luteoviruses. This primer pair generated specific PCR fragments of about 530 bp from extracts of plants infected with potato leafroll virus, beet western yellows virus, or New York barley yellow dwarf virus (BYDV) serotypes MAV, PAV… CONTINUE READING

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