Use of green fluorescent protein-expressing Aspergillus fumigatus conidia to validate quantitative PCR analysis of air samples collected on filters.

@article{McDevitt2005UseOG,
  title={Use of green fluorescent protein-expressing Aspergillus fumigatus conidia to validate quantitative PCR analysis of air samples collected on filters.},
  author={J. McDevitt and Peter S. J. Lees and William G. Merz and Kellogg J. Schwab},
  journal={Journal of occupational and environmental hygiene},
  year={2005},
  volume={2 12},
  pages={633-40}
}
This study used green fluorescent protein (GFP)-expressing Aspergillus fumigatus conidia to compare quantitative PCR (qPCR) enumeration with direct epifluorescent microscopic filter counts of conidia collected on filters in a test chamber. In separate experiments this study initially compared white versus fluorescent light microscopy for counting A. fumigatus conidia, then compared fluorescent microscopy counting of corresponding filter halves, and finally compared qPCR enumeration to counting… CONTINUE READING