Use of genetically ablated stigmas for the isolation of genes expressed specifically in the stigma epidermis

@article{Kang2001UseOG,
  title={Use of genetically ablated stigmas for the isolation of genes expressed specifically in the stigma epidermis},
  author={Yourha Kang and June B Nasrallah},
  journal={Sexual Plant Reproduction},
  year={2001},
  volume={14},
  pages={85-94}
}
In crucifers, the epidermal (papillar) cell of the stigma is uniquely specialized to allow the adhesion, hydration, and germination of pollen grains. In order to isolate genes expressed specifically in papillar cells, we compared the distribution of transcripts expressed in wild-type Brassica stigmas to those expressed in transgenic stigmas whose papillar cells were genetically ablated by expression of a cellular toxin under the control of a papillar cell-specific promoter. This analysis… CONTINUE READING

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