Use of gene targeting for compromising energy homeostasis in neuro-muscular tissues: the role of sarcomeric mitochondrial creatine kinase.

@article{Steeghs1997UseOG,
  title={Use of gene targeting for compromising energy homeostasis in neuro-muscular tissues: the role of sarcomeric mitochondrial creatine kinase.},
  author={Karen Steeghs and Arend Heerschap and Alex de Haan and Wim Ruitenbeek and F. J. J. M. Oerlemans and Jan van Deursen and Barbara Perryman and Dirk Pette and Marloes Br{\"u}ckwilder and J. C. M. Koudijs and Paul H. K. Jap and B. Wieringa},
  journal={Journal of neuroscience methods},
  year={1997},
  volume={71 1},
  pages={29-41}
}
We have introduced a single knock-out mutation in the mitochondrial creatine kinase gene (ScCKmit) in the mouse germ line via targeted mutagenesis in mouse embryonic stem (ES) cells. Surprisingly, ScCKmit -/- muscles, unlike muscles of mice with a deficiency of cytosolic M-type creatine kinase (M-CK -/-; Van Deursen et al. (1993) Cell 74, 621-631), display no altered morphology, performance or oxidative phosphorylation capacity. Also, the levels of high energy phosphate metabolites were… CONTINUE READING

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