Use of gene dosage effects for a whole-genome screen to identify Mycobacterium marinum macrophage infection loci.

@article{Park2008UseOG,
  title={Use of gene dosage effects for a whole-genome screen to identify Mycobacterium marinum macrophage infection loci.},
  author={Bonggoo Park and Selvakumar Subbian and Sahar H El-Etr and Suat L. G. Cirillo and Jeffrey David Cirillo},
  journal={Infection and immunity},
  year={2008},
  volume={76 7},
  pages={3100-15}
}
We recently identified two loci, mel1 and mel2, that affect macrophage infection by Mycobacterium marinum. The ability of these loci to confer enhanced infection in trans is presumably due to gene dosage effects since their presence on plasmids increases expression from five- to eightfold. Reasoning that this phenomenon would allow identification of other mycobacterial genes involved in macrophage infection, we conducted a screen of an M. marinum DNA library that provides 2.6-fold coverage of… CONTINUE READING