Use of fused gfp and gus reporters for the recovery of transformed Medicago truncatula somatic embryos without selective pressure

@article{Duque2007UseOF,
  title={Use of fused gfp and gus reporters for the recovery of transformed Medicago truncatula somatic embryos without selective pressure},
  author={Ana Sofia Rold{\~a}o Lopes Amaral Duque and Susana Ara{\`u}jo and Matilde Ata{\'i}de Cordeiro and Dulce Maria Metelo Fernandes dos Santos and Manuel Pedro Salema Fevereiro},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2007},
  volume={90},
  pages={325-330}
}
We developed an alternative methodology for in vitro selection of transgenic Medicago truncatula cv. Jemalong plants using a bifunctional construct in which the coding sequences for the green fluorescent protein (GFP) and the β-glucuronidase protein (GUS) are fused. An Agrobacterium-mediated transformation protocol was used followed by regeneration via somatic embryogenesis in the dark, to avoid the synthesis and the consequent autofluorescence of chlorophyll. This method is a clear advantage… CONTINUE READING