Use of focused acoustics for cell disruption to provide ultra scale-down insights of microbial homogenization and its bioprocess impact--recovery of antibody fragments from rec E. coli.

@article{Li2012UseOF,
  title={Use of focused acoustics for cell disruption to provide ultra scale-down insights of microbial homogenization and its bioprocess impact--recovery of antibody fragments from rec E. coli.},
  author={Qiang Li and Jean P Aucamp and Alison Tang and Alex Chatel and M. J. Hoare},
  journal={Biotechnology and bioengineering},
  year={2012},
  volume={109 8},
  pages={2059-69}
}
An ultra scale-down (USD) device that provides insight of how industrial homogenization impacts bioprocess performance is desirable in the biopharmaceutical industry, especially at the early stage of process development where only a small quantity of material is available. In this work, we assess the effectiveness of focused acoustics as the basis of an USD cell disruption method to mimic and study high-pressure, step-wise homogenization of rec Escherichia coli cells for the recovery of an… CONTINUE READING