Use of fluorescence resonance energy transfer to investigate the conformation of DNA substrates bound to the Klenow fragment.

@article{Furey1998UseOF,
  title={Use of fluorescence resonance energy transfer to investigate the conformation of DNA substrates bound to the Klenow fragment.},
  author={W. Scott Furey and Catherine M. Joyce and Mark A. Osborne and David Klenerman and James A Peliska and Shankar Balasubramanian},
  journal={Biochemistry},
  year={1998},
  volume={37 9},
  pages={2979-90}
}
Fluorescence resonance energy transfer (FRET) has been used to investigate the conformation of the single stranded region for a series of fluorescent DNA template-primers bound to the Klenow fragment (KF) of Escherichia coli DNA polymerase I. Fluorescent derivatives of template-primer DNA, modified with tetramethylrhodamine (TMR), served as energy transfer acceptors to the donor fluorescein fluorophore used to modify cysteine 751 in the double mutant KF (S751C, C907S). Design of the template… CONTINUE READING