Fluorescein angiography (FA) is a valuable tool for the assessment of fundus alterations secondary to intraocular inflammation, especially in intermediate, posterior, or panuveitis. Like other imaging techniques, FA patterns are not specific for any inflammatory condition and have a low specificity in differential diagnosis. However, this technique enables visualization and investigation of different structures involved in posterior uveitis. It also provides information on the site and extent of uveitis. FA does not allow accurate study of the choroid and the choriocapillaris, which are better analyzed by indocyanine green angiography (ICG). The role of FA in the monitoring of fundus lesions and some representative examples will be illustrated in this review.