Use of flow cytometry to measure the interaction between Escherichia coli heat-stable enterotoxin and its intestinal receptor in mice.

@article{AlMajali1999UseOF,
  title={Use of flow cytometry to measure the interaction between Escherichia coli heat-stable enterotoxin and its intestinal receptor in mice.},
  author={Ahmad Mahmoud Al-Majali and Joseph P. Robinson and Elikplimi K. Asem and Carlton H. Lamar and Melissa J. Freeman and A. Mahdi Saeed},
  journal={Journal of immunological methods},
  year={1999},
  volume={222 1-2},
  pages={65-72}
}
Binding of Escherichia coli heat-stable enterotoxin (STa) to its putative receptor on the brush border membrane of enterocytes is a prerequisite for the induction of diarrhea in infected humans and animals. Humans and animals of different ages vary in their susceptibility to the effect STa, perhaps due to the difference in STa interaction with its intestinal receptor. Flow cytometry was compared to indirect immunofluorescence and 125I-STa binding assays to measure the STa-enterocytes receptor… CONTINUE READING