Use of flow cytometry to follow the physiological states of microorganisms in cider fermentation processes.

@article{Herrero2006UseOF,
  title={Use of flow cytometry to follow the physiological states of microorganisms in cider fermentation processes.},
  author={Monica V. Herrero and Covadonga Quir{\'o}s and Luis A. Garc{\'i}a and Mario D{\'i}az},
  journal={Applied and environmental microbiology},
  year={2006},
  volume={72 10},
  pages={6725-33}
}
The flow cytometry (FC) technique used with certain fluorescent dyes (ChemChrome V6 [CV6], DRAQ5, and PI) has proven useful to label and to detect different physiological states of yeast and malolactic bacterium starters conducting cider fermentation over time (by performing sequential inoculation of microorganisms). First, the technique was tested with pure cultures of both types of microorganisms grown in synthetic media under different induced stress conditions. Metabolically active cells… CONTINUE READING

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