Use of electrospray ionization liquid chromatography-mass spectrometry to study the role of CYP2D6 in the in vitro metabolism of 5-hydroxytryptamine receptor antagonists.

P. Sanwald; M. David; J. Dow

DOI:10.1016/0378-4347(95)00446-7

Corpus ID: 26554262

Use of electrospray ionization liquid chromatography-mass spectrometry to study the role of CYP2D6 in the in vitro metabolism of 5-hydroxytryptamine receptor antagonists.

@article{Sanwald1996UseOE,
  title={Use of electrospray ionization liquid chromatography-mass spectrometry to study the role of CYP2D6 in the in vitro metabolism of 5-hydroxytryptamine receptor antagonists.},
  author={P. Sanwald and M. David and J. Dow},
  journal={Journal of chromatography. B, Biomedical applications},
  year={1996},
  volume={678 1},
  pages={
          53-61
        }
}

P. Sanwald, M. David, J. Dow

Published 1996

Chemistry, Medicine

Journal of chromatography. B, Biomedical applications

An electrospray ionization liquid chromatographic-mass spectrometric (ESI-LC-MS) method has been developed to study the involvement of the cytochrome P450 isoenzyme CYP2D6 in the in vitro metabolism of the indole containing 5-hydroxytryptamine (5-HT(3)++) receptor antagonists tropisetron, ondansetron and dolasetron in human liver microsomes. Compounds were eluted using linear gradients of acetonitrile-20 mM ammonium acetate, solvent A, (10:90, v/v) (pH 6.0) and solvent B, (60:40, v/v) (pH 6.0… CONTINUE READING

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