Use of electrophoretic mobility to determine the secondary structure of a small antisense RNA.

@article{Jacques1991UseOE,
  title={Use of electrophoretic mobility to determine the secondary structure of a small antisense RNA.},
  author={Judith Jacques and M M Susskind},
  journal={Nucleic acids research},
  year={1991},
  volume={19 11},
  pages={2971-7}
}
Natural antisense RNAs have stem-loop (hairpin) secondary structures that are important for their function. The sar antisense RNA of phage P22 is unusual: the 3' half of the molecule forms an extensive stem-loop, but potential structures for the 5' half are not predicted to be thermodynamically stable. We devised a novel method to determine the secondary… CONTINUE READING