Use of duplex polymerase chain reaction (duplex-PCR) technique to identify bovine and water buffalo milk used in making mozzarella cheese.

@article{Rea2001UseOD,
  title={Use of duplex polymerase chain reaction (duplex-PCR) technique to identify bovine and water buffalo milk used in making mozzarella cheese.},
  author={Stephen Rea and Koichi Chikuni and Raffaella Branciari and R S Sangamayya and David Ranucci and P. Avellini},
  journal={The Journal of dairy research},
  year={2001},
  volume={68 4},
  pages={689-98}
}
Molecular biology techniques have been used for species identification in food of animal origin in relatively recent years. A polymerase chain reaction (PCR) based method, the multiplex PCR, was recently applied to species identification in meat and meat products. It allows co-amplification of separate regions of a single gene or specific fragments, each typical of a different animal species in a single PCR reaction, using different pairs of primers in the same reaction mix. In the present… CONTINUE READING
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