Use of dual marker transposons to identify new symbiosis genes in Rhizobium

  title={Use of dual marker transposons to identify new symbiosis genes in Rhizobium},
  author={Chuanwu Xi and Gunter Dirix and Johan Hofkens and Frans C. de Schryver and Jos Vanderleyden and Dr. Jules Michiels},
  journal={Microbial Ecology},
Rhizobium etli elicits nitrogen-fixing nodules on the roots of Phaseolus vulgaris. Using a composite dual-marker mini-Tn5 transposon carrying combinations of a constitutively expressed gfp gene and a promoterless gusA gene, we identified novel genes required for an efficient symbiosis. The induction of the gusA gene was used to determine the expression level of the different target genes under conditions partly mimicking the symbiotic environment ex planta. The green fluorescence was used to… CONTINUE READING


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