Use of degradation tags to control protein levels in the Cyanobacterium Synechocystis sp. Strain PCC 6803.

Abstract

We generated a collection of ssrA-based C-terminal protein degradation tags with different degradation strengths. The steady-state fluorescence levels of different enhanced yellow fluorescent protein (eYFP) tag variants in a Synechocystis sp. indicated a tunable range from 1% to 50% of untagged eYFP. 
DOI: 10.1128/AEM.03741-12

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Cite this paper

@article{Landry2013UseOD, title={Use of degradation tags to control protein levels in the Cyanobacterium Synechocystis sp. Strain PCC 6803.}, author={Brian P. Landry and Jana St{\"o}ckel and Himadri B. Pakrasi}, journal={Applied and environmental microbiology}, year={2013}, volume={79 8}, pages={2833-5} }