Use of cultured renal epithelial cells for the study of cisplatin toxicity.

@article{Fukuishi1989UseOC,
  title={Use of cultured renal epithelial cells for the study of cisplatin toxicity.},
  author={Nobuyuki Fukuishi and Munekazu Gemba},
  journal={Japanese journal of pharmacology},
  year={1989},
  volume={50 2},
  pages={247-9}
}
The nephrotoxicity of cisplatin was evaluated in an in vitro system with an established cell line of normal rat kidney, NRK-52E. Leakage of enzymes from the cells into the culture medium increased when they were exposed to 1 microM of cisplatin for 72 hr. The level of lipid peroxides increased in the cells after 48 hr of exposure to cisplatin; the increase was more rapid than the enzyme leakage. This culture system can be used to evaluate drug-induced nephrotoxicity and its mechanism.