Use of cultured human epidermal keratinocytes for allografting burns and conditions for temporary banking of the cultured allografts.

@article{BolvarFlores1990UseOC,
  title={Use of cultured human epidermal keratinocytes for allografting burns and conditions for temporary banking of the cultured allografts.},
  author={J Bol{\'i}var-Flores and E Poumian and Meytha Marsch-Moreno and Georgina Montes de Oca and Walid Kuri-Harcuch},
  journal={Burns : journal of the International Society for Burn Injuries},
  year={1990},
  volume={16 1},
  pages={
          3-8
        }
}

Treatment of burns and donor sites with human allogeneic keratinocytes grown on acellular pig dermis

TLDR
Rec recombined human/pig skin (RHPS) composed of human keratinocytes cultured on cell‐free pig dermis is proved that RHPS can serve as an effective, ready to use keratinocyte delivery system when applied‘upside‐down', i.e. with epidermal cells facing the wound surface.

Human allogeneic keratinocytes cultured on acellular xenodermis: the use in healing of burns and other skin defects.

TLDR
The tissue engineered skin should be composed of both dermal and epidermal layers and can be used as temporary cover for donor sites, small to medium leg ulcers and other skin defects.

Frozen cultured sheets of human epidermal keratinocytes enhance healing of full-thickness wounds in mice

TLDR
Most wounds treated with frozen cultures completely healed after 10 days, whereas most control wounds required 16 days, and the accelerated reepithelialization did not depend on the presence of proliferative human keratinocytes in the frozen cultures.

Prevention of burn wound conversion by allogeneic keratinocytes cultured on acellular xenodermis

TLDR
RHPS, consisting of human allogeneic keratinocytes cultured on acellular pig dermis, has skin-like consistency and therefore optimal adhesiveness to the wound, and more than 70% of early excised or deeply dermabraded wounds grafted with RHPS healed in the course of one week after keratinocyte transplantation.

Frozen human epidermal allogeneic cultures promote rapid healing of facial dermabrasion wounds.

TLDR
Epidermal allogenic cultures, preserved by freezing, promoted significantly faster reepithelization and reduced pain intensity of dermabraded facial wounds, suggesting that they could be used routinely to improve the recovery from dermABrasion.

Frozen allogeneic human epidermal cultured sheets for the cure of complicated leg ulcers.

TLDR
The use of frozen human allogeneic epidermal cultures is a safe and effective treatment for venous or diabetic ulcers, even those with tendon exposure.

Human cultured epidermis accelerates wound healing regardless of its viability in a diabetic mouse model

TLDR
The promoting effect of allo-CE on wound healing does not depend on cell viability and Lyophilized CEs may be a suitable wound dressing with a long storage period at room temperature.

Title Human cultured epidermis accelerates wound healing regardless of its viability in a diabetic mouse model

TLDR
The promoting effect of allo-CE on wound healing does not depend on cell viability and Lyophilized CEs may be a suitable wound dressing with a long storage period at room temperature.

Tissue-Engineered Skin

TLDR
Tissue-engineered skin is a significant advance in the field of wound healing and was developed due to limitations associated with the use of autografts but the potential risks and benefits need to be further evaluated in clinical trials.

References

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Long-term survival and immunological tolerance of human epidermal allografts produced in culture.

TLDR
Findings show that Langerhans cell and other class-II-antigen-bearing cell-depleted cultured epidermal allografts, are tolerated in unrelated recipients, and may serve as a skin substitute in patients with large wounds or burns.

Grafting of cultured allogeneic epidermis on second- and third-degree burn wounds on 26 patients.

TLDR
After being grafted with cultured allogeneic epidermal cells, superficial wounds, e.g., donor sites, healed within 7 days, compared to 14 days for mirror image control sites, and deep second-degree burn wounds, which were excised before grafting with cultured cells healed in a mean time of 10 days.

Growth and differentiation of human epidermal cultures used as auto‐ and allografts in humans

TLDR
The analysis of keratins and examination of various keratinocyte membrane antigens by immunofluorescence indicated that full terminal epithelial differentiation was only achieved after in vivo transplantation of the cultured epidermis.

Progressive replacement of human cultured epithelial allografts by recipient cells as evidenced by HLA class I antigens expression.

TLDR
A progressive replacement by recipient cells of the grafted keratinocytes is shown, related to the fact that, when placed on such superficial dermal wounds, the allografts are likely colonized by epithelial cells proliferating from residual recipient dermal appendages.

Combined Burn Therapy Utilizing Immediate Skin Allografts and 0.5% AgNO3

TLDR
It appears that the metabolic demands inherent in maintenance of homeostasis and in repair of damaged tissue, if carried on over a long period of time, may approach a magnitude with which the host cannot cope.

Growth of cultured human epidermal cells into multiple epithelia suitable for grafting.

TLDR
It will be shown here that large amounts of cultured epithelium can be generated from a small piece of epidermis in a short time.

Formation of epidermis by serially cultivated human epidermal cells transplanted as an epithelium to athymic mice.

TLDR
The human origin of the grafts was demonstrated with a species-specific antiserum to a precursor protein of the cross-linked envelope and such grafts formed epidermis complete with stratum corneum.