Use of cultured cells to study the relationship between arachidonic acid and endothelium-derived relaxing factor.

@article{Johns1988UseOC,
  title={Use of cultured cells to study the relationship between arachidonic acid and endothelium-derived relaxing factor.},
  author={Roger A Johns and Nicholas J. Izzo and Pamela Milner and Alex L. Loeb and Michael J G Peach},
  journal={The American journal of the medical sciences},
  year={1988},
  volume={295 4},
  pages={
          287-92
        }
}
We have used mixed- and co-cultures of endothelial and vascular smooth muscle cells to investigate the role of phospholipase activation and arachidonic acid metabolites in the production of endothelium-derived relaxing factor (EDRF). Inhibition of phospholipase A2 with para-bromophenacyl bromide, dexamethasone or quinacrine, alone or in combination, blocked arachidonate release by 50%-60% but had no effect on EDRF production as assessed by cyclic GMP accumulation in mixed- or co-cultures of… CONTINUE READING