Use of competitive dead-end inhibitors to determine the chemical mechanism of action of yeast alcohol dehydrogenase

@article{Leskovac1998UseOC,
  title={Use of competitive dead-end inhibitors to determine the chemical mechanism of action of yeast alcohol dehydrogenase},
  author={Vladimir Leskovac and Svetlana Trivi{\'c} and Bruce M. Anderson},
  journal={Molecular and Cellular Biochemistry},
  year={1998},
  volume={178},
  pages={219-227}
}
In this work, we have postulated a comprehensive and unified chemical mechanism of action for yeast alcohol dehydrogenase (EC 1.1.1.1, constitutive, cytoplasmic), isolated from Saccharomyces cerevisiae. The chemical mechanism of yeast enzyme is based on the integrity of the proton relay system: His-51....NAD+....Thr-48....R.CH2OH(H2>O)....Zn<math>++, stretching from His-51 on the surface of enzyme to the active site zinc atom in the substrate-binding site of enzyme. Further, it is based on… CONTINUE READING

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