Use of column temperature to optimize carotenoid isomer separation by C30 high performance liquid chromatography

@inproceedings{Bhm2001UseOC,
  title={Use of column temperature to optimize carotenoid isomer separation by C30 high performance liquid chromatography},
  author={Volker B{\"o}hm},
  year={2001}
}
  • Volker Böhm
  • Published 2001
  • Chemistry
  • Five xanthophylls and six carotenes were eluted from a YMC C 30 column with a binary mobile phase gradient consisting of methyl tert-butyl ether and methanol. The effect of column temperature on the separation was tested between 15°C and 25°C. A mixture of prominent lycopene isomers was additionally analyzed to optimize their separation in relation to the main xanthophylls and carotenes. At column temperatures below 20°C (15Z)-β-carotene co-eluted with echinenone, a frequently used internal… CONTINUE READING

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