Use of coisogenic host blastocysts for efficient establishment of germline chimeras with C57BL/6J ES cell lines.

Abstract

Gene targeting in embryonic stem (ES) cells allows the production of mice with specified genetic mutations. Currently, germline-competent ES cell lines are available from only a limited number of mouse strains, and inappropriate ES cell/host blastocyst combinations often restrict the efficient production of gene-targeted mice. Here, we describe the… (More)

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@article{SchusterGossler2001UseOC, title={Use of coisogenic host blastocysts for efficient establishment of germline chimeras with C57BL/6J ES cell lines.}, author={Karin Schuster-Gossler and Andrew W P Lee and Charles P. Lerner and Hugo J. Parker and V W Dyer and Valerie E Scott and Andrea Gossler and Jackie Conover}, journal={BioTechniques}, year={2001}, volume={31 5}, pages={1022-4, 1026} }