Use of chromatin immunoprecipitation to clone novel E2F target promoters.

  title={Use of chromatin immunoprecipitation to clone novel E2F target promoters.},
  author={Amy S. Weinmann and Stephanie M. Bartley and Tong Zhang and Michael Q Zhang and Peggy J. Farnham},
  journal={Molecular and cellular biology},
  volume={21 20},
We have taken a new approach to the identification of E2F-regulated promoters. After modification of a chromatin immunoprecipitation assay, we cloned nine chromatin fragments which represent both strong and weak in vivo E2F binding sites. Further characterization of three of the cloned fragments revealed that they are bound in vivo not only by E2Fs but also by members of the retinoblastoma tumor suppressor protein family and by RNA polymerase II, suggesting that these fragments represent… CONTINUE READING
Highly Influential
This paper has highly influenced 13 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS
161 Citations
50 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 161 extracted citations


Publications referenced by this paper.
Showing 1-10 of 50 references

Binding specificity of E2F and pocket protein family members in living cells

  • J. Wells, K. E. Boyd, S. M. Bartley, P. J. Farnham
  • Mol . Cell . Biol .
  • 2000
3 Excerpts

Similar Papers

Loading similar papers…