Use of caffeine as a probe for rapid determination of cytochrome P-450 CYP1A2 activity in humans.

Abstract

AIM To develop a rapid HPLC method for the determination of cytochrome P-450 CYP1A2 activity. METHODS A 300-microL plasma was prepared by extraction with 5-mL chloroform/isopropanol (9:1), and beta-hydroxyletheophylline was added as internal standard (IS). Samples were separated on an ODS column by a gradient elution system, of which mobile phase… (More)

Topics

  • Presentations referencing similar topics