Use of bone marrow somatic cell hybrid lines to generate monoclonal antibodies specifically reactive with rare marrow cells.

@article{Hasthorpe1990UseOB,
  title={Use of bone marrow somatic cell hybrid lines to generate monoclonal antibodies specifically reactive with rare marrow cells.},
  author={Suzanne Hasthorpe and J T Rogerson and Susannah L Green and Julie M. A. Radley},
  journal={Experimental hematology},
  year={1990},
  volume={18 3},
  pages={223-7}
}
The technique of somatic cell hybridization has been applied to obtain new sources of immunogen for monoclonal antibody production. A marrow population enriched for rare and undifferentiated cells was hybridized with A9 cells. Mature cells were depleted by using mice at 8 days following 5-fluorouracil treatment and by using a sorting procedure. Selection of… CONTINUE READING