Use of biotechnologies for the conservation of plant biodiversity

  title={Use of biotechnologies for the conservation of plant biodiversity},
  author={Florent Engelmann},
  journal={In Vitro Cellular \& Developmental Biology - Plant},
  • F. Engelmann
  • Published 1 February 2011
  • Environmental Science
  • In Vitro Cellular & Developmental Biology - Plant
In vitro techniques are very useful for conserving plant biodiversity, including (a) genetic resources of recalcitrant seed and vegetatively propagated species, (b) rare and endangered plant species and (c) biotechnology products such as elite genotypes and genetically engineered material. Explants from recalcitrant seed and vegetatively propagated species can be efficiently collected under field conditions using in vitro techniques. In vitro culture techniques ensure the production and rapid… 
Biotechnology and Conservation of Plant Biodiversity
The number of plant species for which cryopreservation techniques are established and validated on a large range of genetically diverse accessions is increasing steadily, and the number of animal and vegetable species targeted for conservation is also increasing steadily.
In Vitro Conservation Through Slow-Growth Storage
In vitro slow-growth storage enables a possible solution for mid- to long-term conservation of plant materials in limited space and at reduced costs too.
In Vitro Conservation and Cryopreservation of Clonally Propagated Horticultural Species
Although large-scale, routine application of cryopreservation is still limited to a few laboratories, there are an ever-increasing number of publications reporting successful protocols in many horticultural species, due to advances in development of more ‘generic’ new-age cryop Reservation protocols like droplet-vitrification.
Biotechnological Interventions for Conservation and Multiplication of Threatened Medicinal Plants
In this chapter an attempt has been made to review the state of art in these species in relation with multiplication and conservation using biotechnological means.
In vitro techniques for grapevine germplasm conservation
High-scale in vitro conservation of grapevine germplasm is feasible and may safeguard valuable biodiversity, although promising, cryopreservation requires further studies for protocol optimization.
Cryopreservation of Woody Crops: The Avocado Case
Ex situ conservation, namely field repositories and in vitro storage for some of the world’s most important crops, are looked at, focusing on conservation strategies for avocado.
Biotechnological Approaches for Conservation of the Genus Pistacia
Micropropagation, micrografting, and somatic embryogenesis procedures are described here in addition to in vitro conservation and cryopreservation in Pistacia species to prevent possible genetic erosion.
Cryopreservation of plant genetic resources: A legacy for humanity
A successful cryopreservation protocol depends on survival, regeneration and genetic stability of plant materials, especially during plant regeneration by the use of plant growth regulators.
Biotechnology for Endangered Plant Conservation
In vitro techniques for plant conservation include photoautotrophic micropropagation, somatic embryogenesis, cell culture and embryo rescue techniques, as well as in vitro cold storage and cryopreservation methods and their contribution toward an integrated system to conserve endangered plant species are presented.


The contribution of in vitro technology and cryogenic storage to conservation of indigenous plants (Review)
The role of in vitro and cryogenic research initiatives in botanic gardens for plant biodiversity conservation and restoration is discussed, using examples of successful ex situ conservation through tissue-culture and cryogen-storage research.
Plant cryopreservation: Progress and prospects
  • F. Engelmann
  • Biology
    In Vitro Cellular & Developmental Biology - Plant
  • 2004
There are a growing number of examples where cryopreservation is employed on a large scale for different types of materials, including seeds with orthodox and intermediate storage behaviour, dormant buds, pollen, biotechnology products, and apices sampled from in vitro plantlets of vegetatively propagate species.
Cryopreservation of Bryophytes and Ferns
Cryostorage of tissues or spores of endangered nonseed plants can provide a back up to threatened wild populations, while genetically valuable research lines can be maintained in a stable and cost-effective fashion using liquid nitrogen (LN) storage.
In vitro conservation of tropical plant germplasm-a review
In vitro medium term conservation of tropical plant germplasm is used routinely in many laboratories. Growth reduction is achieved by modifying various parameters, such as temperature, culture
Conservation In vitro of threatened plants—Progress in the past decade
Photautotrophic micropropagation has the potential for development as a routine method for the in vitro conservation of endangered plants and the potential applications of cryopreservation are significant in this area.
Application of cryopreservation protocols at a clonal genebank.
A long-term base collection of pear meristems and pollen in liquid nitrogen is initiated, furthering the thought that cryopreservation should be used rather than discussed, and making it possible to protect germplasm collections through base (long-term back-up) storage inLiquid nitrogen.
In vitro conservation of plant genetic resources
The conservation of biodiversity is widely recognized as a high priority area for attention in the ongoing debate linking environment and development [1,2]. In particular, the conservation of plant
Cost Efficiency of Cryopreservation as a Long-Term Conservation Method for Coffee Genetic Resources
This study compared the costs of maintaining one of the world’s largest coffee collections with those of establishing a coffee cryo-collection at the Centro Agronomico Tropical de Investigacion y Ensenanza (CATIE) in Costa Rica and showed that cryopreservation costs less (in perpetuity per accession) than conservation in fi eld genebanks.
Cryopreservation of Embryos
For the last decade, plant cell cultures have become subjects of intensive research with emphasis on important biotechnological applications such as large scale production of clonal plants, genetic