Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli.

@article{Murphy1998UseOB,
  title={Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli.},
  author={Kenan C. Murphy},
  journal={Journal of bacteriology},
  year={1998},
  volume={180 8},
  pages={2063-71}
}
Replacement of Escherichia coli's RecBCD function with phage lambda's Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is highlighted by gene replacement with a PCR-generated… CONTINUE READING