Use of bacteriophage MS2 as an internal control in viral reverse transcription-PCR assays.

Abstract

Diagnostic systems based on reverse transcription (RT)-PCR are widely used for the detection of viral genomes in different human specimens. The application of internal controls (IC) to monitor each step of nucleic acid amplification is necessary to prevent false-negative results due to inhibition or human error. In this study, we designed various real-time… (More)

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Cite this paper

@article{Dreier2005UseOB, title={Use of bacteriophage MS2 as an internal control in viral reverse transcription-PCR assays.}, author={Jens P Dreier and Melanie St{\"{o}rmer and Knut Kleesiek}, journal={Journal of clinical microbiology}, year={2005}, volume={43 9}, pages={4551-7} }