Use of autologous tissue engineered skin to treat porcine full-thickness skin defects.


OBJECTIVE To explore a feasible method to repair full-thickness skin defects utilizing tissue engineered techniques. METHODS The Changfeng hybrid swines were used and the skin specimens were cut from the posterior limb girdle region, from which the keratinocytes and fibroblasts were isolated and harvested by trypsin, EDTA, and type II collagenase. The… (More)


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