Use of artificial DNA with multiple probe sites as reference DNA templates for quantitative real-time PCR to examine methanogen communities.

Abstract

Isolation of reference DNA templates for quantitative real-time PCR assays is an expensive, labor-intensive and time-consuming process if they are not readily available. Two artificial DNA templates with multiple probe sites were designed for quantifying methanogens and their 10 subgroups, based on the methyl coenzyme M reductase gene (mcrA). Their standards were comparable to each other. PCR amplification efficiencies (cycle vs. cumulative fluorescence) of the artificial DNAs were also comparable to those of the observed methanogen groups from anaerobic digesters. The artificial templates can be alternatives to the actual references.

DOI: 10.1080/10934529.2013.728915

Statistics

02004002014201520162017
Citations per Year

120 Citations

Semantic Scholar estimates that this publication has 120 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Kim2013UseOA, title={Use of artificial DNA with multiple probe sites as reference DNA templates for quantitative real-time PCR to examine methanogen communities.}, author={Tae Gwan Kim and Taewoo Yi and Kyung-suk Cho}, journal={Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering}, year={2013}, volume={48 4}, pages={417-21} }