Use of aphid stylectomy and RT-PCR for the detection of transporter mRNAs in sieve elements.

@article{DoeringSaad2002UseOA,
  title={Use of aphid stylectomy and RT-PCR for the detection of transporter mRNAs in sieve elements.},
  author={Christian Doering-Saad and H. John Newbury and Jeff Bale and J. Diane Pritchard},
  journal={Journal of experimental botany},
  year={2002},
  volume={53 369},
  pages={631-7}
}
Unmodified samples of barley (Hordeum vulgare) sieve tube sap have been obtained by severing the stylets (stylectomy) of feeding aphids and collecting the exuding liquid. Primers were designed to direct the amplification of a series of specific cDNAs encoding barley proteins selected because of their significance in sieve tube function. mRNA encoding the H(+)/sucrose co-transporter SUT1, a putative aquaporin and the H(+)/ATPase PPA1 were detected in sieve tube sap. These mRNA species appear to… CONTINUE READING

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