Use of anchor protein modules in fluorescence polarisation aptamer assay for ochratoxin A determination.

@article{Samokhvalov2017UseOA,
  title={Use of anchor protein modules in fluorescence polarisation aptamer assay for ochratoxin A determination.},
  author={Alexey V Samokhvalov and Irina V Safenkova and Sergei Alexandrovich Eremin and Anatoly V. Zherdev and Boris B. Dzantiev},
  journal={Analytica chimica acta},
  year={2017},
  volume={962},
  pages={80-87}
}
A new strategy for sensitive fluorescence polarisation (FP) analysis is proposed which uses aptamer as the receptor and anchor protein modules as the enhancers by including the aptamers in complexes with protein modules. This approach is based on increasing the size differences of bound and unbound fluorophores. The strategy was applied in an ochratoxin A (ОТА) assay with the competitive binding of fluorophore-labelled and free OTA with aptamer-based receptors. We showed that the binding of… CONTINUE READING

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