Use of an intravenous microdose of 14C-labeled drug and accelerator mass spectrometry to measure absolute oral bioavailability in dogs; cross-comparison of assay methods by accelerator mass spectrometry and liquid chromatography-tandem mass spectrometry.

@article{Miyaji2009UseOA,
  title={Use of an intravenous microdose of 14C-labeled drug and accelerator mass spectrometry to measure absolute oral bioavailability in dogs; cross-comparison of assay methods by accelerator mass spectrometry and liquid chromatography-tandem mass spectrometry.},
  author={Yoshihiro Miyaji and Tomoko Ishizuka and Kenji Kawai and Yoshimi Hamabe and Teiji Miyaoka and T Oh-hara and Toshihiko Ikeda and Atsushi Kurihara},
  journal={Drug metabolism and pharmacokinetics},
  year={2009},
  volume={24 2},
  pages={
          130-8
        }
}
A technique utilizing simultaneous intravenous microdosing of (14)C-labeled drug with oral dosing of non-labeled drug for measurement of absolute bioavailability was evaluated using R-142086 in male dogs. Plasma concentrations of R-142086 were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and those of (14)C-R-142086 were measured by accelerator mass spectrometry (AMS). The absence of metabolites in the plasma and urine was confirmed by a single radioactive peak of the… 

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