Use of an automated multiple-locus, variable-number tandem repeat-based method for rapid and high-throughput genotyping of Staphylococcus aureus isolates.

@article{Franois2005UseOA,
  title={Use of an automated multiple-locus, variable-number tandem repeat-based method for rapid and high-throughput genotyping of Staphylococcus aureus isolates.},
  author={Patrice François and Antoine Huyghe and Yvan Charbonnier and Manuela Teixeira Bento and S{\'e}bastien Herzig and Ivan Topolski and B{\'e}n{\'e}dicte Fleury and Daniel Lew and Pierre Vaudaux and Stephan Harbarth and Willem van Leeuwen and Alex van Belkum and Dominique S. Blanc and Didier Pittet and Jacques Schrenzel},
  journal={Journal of clinical microbiology},
  year={2005},
  volume={43 7},
  pages={3346-55}
}
Fast and reliable genotyping methods that allow real-time epidemiological surveillance would be instrumental to monitoring of the spread of methicillin-resistant Staphylococcus aureus. We describe an automated variable-number tandem repeat-based method for the rapid genotyping of Staphylococcus aureus. Multiplex PCR amplifications with eight primer pairs that target gene regions with variable numbers of tandem repeats were resolved by microcapillary electrophoresis and automatically assessed by… CONTINUE READING