Use of an albumin-binding domain for the selective immobilisation of recombinant capture antibody fragments on ELISA plates.

Abstract

A small albumin-binding domain (ABD) of 46 amino acids derived from streptococcal protein G was employed for the directed attachment of recombinant immunoglobulin (Ig) fragments to microtitre plates that had been coated with human serum albumin (HSA). Generic vectors were constructed in order to produce the Fv or Fab fragments fused with the ABD in… (More)

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Cite this paper

@article{Koenig1998UseOA, title={Use of an albumin-binding domain for the selective immobilisation of recombinant capture antibody fragments on ELISA plates.}, author={T. Koenig and Arne Skerra}, journal={Journal of immunological methods}, year={1998}, volume={218 1-2}, pages={73-83} }