Use of amino acid analogue-resistant cell lines for selection of Nicotiana sylvestris somatic cell hybrids

@article{White1979UseOA,
  title={Use of amino acid analogue-resistant cell lines for selection of Nicotiana sylvestris somatic cell hybrids},
  author={Derek William Richard White and Indra K. Vasil},
  journal={Theoretical and Applied Genetics},
  year={1979},
  volume={55},
  pages={107-112}
}
Nicotiana sylvestris cell lines resistant to the amino acid analogues S-2-aminoethyl-cysteine (AECR), or 5-methyl-tryptophan (5MTR), were isolated in suspension culture. Assuming these resistances to be dominant, we have attempted to determine if such variant cell lines can be used to select double resistant somatic cell hybrids. A total of 1.8 × 104 control calli from mixed AECR and 5MTR protoplasts, and AECR and 5MTR homokaryotic fusions were placed on double analogue selection, but none… CONTINUE READING
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