Use of acridine orange to evaluate chromatin integrity of human spermatozoa in different groups of infertile men.

@article{Gopalkrishnan1999UseOA,
  title={Use of acridine orange to evaluate chromatin integrity of human spermatozoa in different groups of infertile men.},
  author={Kamala Gopalkrishnan and K. S. Hurkadli and Varsha S Padwal and Donta Balaiah},
  journal={Andrologia},
  year={1999},
  volume={31 5},
  pages={277-82}
}
To study the sperm chromatin compactness various methods, such as acidic aniline blue or acridine orange staining, have been applied. Due to its metachromatic properties, acridine orange dye fluoresces green with double- and red with single-stranded DNA. Samples (n = 181) were evaluated and grouped as follows: group I, normal recently fertile; group II, male having female partner with repeated early pregnancy loss; group III, male with varicocele; and group IV in-vitro fertilization and… CONTINUE READING

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