Use of a virulence-associated protein based enzyme-linked immunosorbent assay for Rhodococcus equi serology in horses.

@article{Prescott1996UseOA,
  title={Use of a virulence-associated protein based enzyme-linked immunosorbent assay for Rhodococcus equi serology in horses.},
  author={J F Prescott and Alicia Silvina Fern{\'a}ndez and Vivian M. Nicholson and Marc C. Patterson and J. M. Link a P. M. Yager and Laurent Viel and Greg Perkins},
  journal={Equine veterinary journal},
  year={1996},
  volume={28 5},
  pages={344-9}
}
An enzyme-linked immunosorbent assay (ELISA) was developed against Rhodococcus equi using Triton X-114 detergent extracted whole cell material, in which the virulence associated protein (VapA) predominated. Enzymelinked immunosorbent assay titres corresponded to antibody reacting with VapA on Western blots. There was considerable variation in antibody titres of nonimmunised mares and in the time when the colostrally derived antibody of their foals had declined to low or undetectable titres. In… CONTINUE READING

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