Use of a trypsin-pulse method to study the refolding pathway of ribonuclease.

Abstract

Trypsin pulses, applied after varying times of refolding, have been employed to probe the accessibility of the polypeptide chain of ribonuclease A during the process of refolding. The increase in resistance against proteolytic cleavage was measured by activity assays and by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. The sites of cleavage… (More)

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@article{Lang1986UseOA, title={Use of a trypsin-pulse method to study the refolding pathway of ribonuclease.}, author={Kurt Lang and Franz Xaver Schmid}, journal={European journal of biochemistry}, year={1986}, volume={159 2}, pages={275-81} }